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http://repositorio.inger.gob.mx/jspui/handle/20.500.12100/17274| Title: | Telomerase activity in response to mild oxidative stress |
| metadata.dc.creator: | NORMA EDITH LOPEZ DIAZ GUERRERO GLORIA ERANDI PEREZ FIGUEROA Cintia Mayel Martínez Garduño ADRIANA ALARCON AGUILAR ARMANDO LUNA LOPEZ MARIA CONCEPCION GUTIERREZ RUIZ MINA KONIGSBERG FAINSTEIN |
| Keywords: | BIOLOGÍA Y QUÍMICA;Química;Bioqímica;Enzimología;Metabolismo;Estres oxidativo;BCL-2;Drogras quimioterapéuticas;Oxidative stress;Chemistry;Biochemistry;Chemotherapeutic drugs |
| metadata.dc.date: | 2012 |
| Publisher: | International Federation for Cell Biology International & Wiley |
| Description: | We have analysed telomerase activity to determine whether it can be modified when BCL-2 is endogenously overexpressed in response to a mild oxidative stress treatment as part of a survival mechanism, in contrast with an exogenous bcl-2 overexpression due to a retroviral infection. Endogenous bcl-2 overexpression was induced after a low oxidative insult of H2O2 in mice primary lung fibroblasts and L929 cell, whereas bcl-2 exogenous overexpression was performed using a retroviral infection in L929 cells. Telomerase activity was quantified in Bcl-2 overexpressing cells by the TRAP assay. When the cells were treated with different H2O2 concentrations, only those exposed to 50 μM showed increased telomerase activity. This correlates with BCL-2 expression as part of the endogenous response to mild oxidative stress. Oxidative stress generated during the toxic mechanism of chemotherapeutic drugs might induce BCL-2 increment, enhancing telomerase activity and reactivating the oncogenic process. Clinical trials should take into consideration the possibility of telomerase activation following increased BCL-2 expression when treating patients with ROS (reactive oxygen species) generation by anti-cancer drugs. |
| URI: | http://repositorio.inger.gob.mx/jspui/handle/20.500.12100/17274 |
| Appears in Collections: | 1. Artículos |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Cell Biology International (1095-8355) Vol. 36 (2012).pdf | 302.23 kB | Adobe PDF | View/Open |