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Oxidative damage and antioxidant defense in thymus of malnourished lactating rats

dc.rights.licensehttp://creativecommons.org/licenses/by/4.0es_MX
dc.creatorGRACIELA GAVIA GARCIAes_MX
dc.creatorHAYDEE GONZALEZ MARTINEZes_MX
dc.creatorANGEL MILIAR GARCIAes_MX
dc.creatorEDMUNDO BONILLA GONZALEZes_MX
dc.creatorMARIA DE LOS ANGELES ROSAS TREJOes_MX
dc.creatorMINA KONIGSBERG FAINSTEINes_MX
dc.creatorORALIA NAJERA MEDINAes_MX
dc.creatorARMANDO LUNA LOPEZes_MX
dc.creatorMARIA CRISTINA GONZALEZ TORRESes_MX
dc.date2015
dc.date.accessioned2021-11-01T18:46:58Z
dc.date.available2021-11-01T18:46:58Z
dc.identifier.urihttp://repositorio.inger.gob.mx/jspui/handle/20.500.12100/17302
dc.descriptionObjective Malnutrition has been associated with oxidative damage by altered antioxidant protection mechanisms. Specifically, the aim of this study was to evaluate oxidative damage (DNA and lipid) and antioxidant status (superoxide dismutase [SOD], glutathione peroxidase [GPx], and catalase [CAT] mRNA, and protein expression) in thymus from malnourished rat pups. Methods Malnutrition was induced during the lactation period by the food competition method. Oxidative DNA damage was determined quantifying 8-oxo-7, 8-dihydro-2'-deoxyguanosine adduct by high-performance liquid chromatography. Lipid peroxidation was assessed by the formation of thiobarbituric acid-reactive substances. Levels of gene and protein expression of SOD, GPx, and CAT were evaluated by real-time polymerase chain reaction and Western blot, respectively. Antioxidant enzyme activities were measured spectrophotometrically. Results Oxidative DNA damage and lipid peroxidation significantly increased in second-degree (MN-2) and third-degree malnourished (MN-3) rats compared with well-nourished rats. Higher amounts of oxidative damage, lower mRNA expression, and lower relative concentrations of protein, as well as decreased antioxidant activity of SOD, GPx, and CAT were associated with the MN-2 and MN-3 groups. Conclusions The results of this study demonstrated that higher body-weight deficits were related to alterations in antioxidant protection, which contribute to increased levels of damage in the thymus. To our knowledge, this study demonstrated for the first time that early in life, malnutrition leads to increased DNA and lipid oxidative damage, attributable to damaged antioxidant mechanisms including transcriptional and enzymatic activity alterations. These findings may contribute to the elucidation of the causes of previously reported thymus dysfunction, and might explain partially why children and adults who have overcome child undernourishment experience immunologic deficiencies.es_MX
dc.formatAdobe PDFes_MX
dc.languageenges_MX
dc.publisherElsevieres_MX
dc.relationhttps://www.sciencedirect.com/science/article/abs/pii/S0899900715002294?via%3Dihubes_MX
dc.relation.requiresSies_MX
dc.rightsAcceso Abiertoes_MX
dc.sourceNutrition (0899-9007) Vol. 31 (2015)es_MX
dc.subjectBIOLOGÍA Y QUÍMICAes_MX
dc.subjectCiencias de la vidaes_MX
dc.subjectEnvejecimientoes_MX
dc.subjectAginges_MX
dc.subjectEstrés oxidativoes_MX
dc.subjectOxidative Stresses_MX
dc.subjectDaño de ADNes_MX
dc.subjectDNA damagees_MX
dc.subjectAntioxidanteses_MX
dc.subjectAntioxidantses_MX
dc.subjectMalnutriciónes_MX
dc.subjectMalnutritiones_MX
dc.titleOxidative damage and antioxidant defense in thymus of malnourished lactating ratses_MX
dc.typeArtículoes_MX
dc.audienceResearcherses_MX
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